Cancer/Metastasis-Associated Genes Screening

- DNA Array Analysis -

Cat. No. SER003

Service

Major Service Items

  • Total RNA extraction & purification

  • RNA quality verification

  • Reverse transcription & labeling

  • Membrane-based cDNA microarrays

  • containing around 550 cancer/metastasis-

  • l  associated gene probes  

  • Hybridization and color development

  • Image scanning, data management & analysis

　

　

　

- Clustering & Classification

<< Example >>

Applications

  • Gene expression analysis of mammalian cells after treatment

  • Clinical studies and identification of cancer/metastasis-associated genes

Background information

  • Microarray is a high-throughput technique for functional genomics studies.  It is a powerful tool for discovery of new experimental clues.

  • Membrane-based cDNA Microarray will be used for gene expression analysis.

  • Technology was transferred from the research laboratory of National TaiwanUniversity (Taiwan).  This microarray system has been adapted and verified for clinical research. Around 200 clinical lung cancer tissues have been analyzed during the last 2 years. Prediction model for metastasis and survival have been set up using annotated cancer/metastasis-associated genes.

  • Commercial software “Avadis” helps to normalization and further exploration of gene expression profile in cells after certain treatments.