G-418.Sulfate
Description:
Aminoglycoside used as a selective agent of transfected mammalian, yeast, plant and bacteria cells. Resistance is conferred by the bacterial gene for aminoglycoside-3'-phophotransferase that can be expressed in eukaryotic cells. Related to Gentamycin.
USAGE:
Geneticin is an aminoglycoside antibiotic similar in structure to gentamycin. It exhibits toxicity towards both eukaryotic and prokaryotic cells. The optimal concentration for selection and maintenance must be determined for each cell line. For bacteria and algae concentrations, 5 ug/ml or less are recommended. Animal cells may require up to 300-500 ug/ml. Typically, resistance is conferred by one of two dominant genes of bacterial origin which can be expressed in eukaryotic cells. Cells that are multiplying will be affected sooner than those that are not. Cells in log phase may require three to seven days for selection. In general, concentrations of approximately 400 ug/ml for selection and 200 ug/ml for maintenance are required for mammalian cells

G418簡介
　　是一種氨基糖甘類抗生素，在分子遺傳試驗中常被用作抗性篩選。它通過抑制轉做子Tn601，Tn5的基因對原核和真核等細胞產生毒素包括細菌、酵母、植物和哺乳動物細胞，也包括原生動物和蠕蟲。當neo基因被整合進真核細胞DNA後，則能啟動neo基因編碼的序列轉錄為ｍＲＮＡ，從而獲得抗性產物的高效表達，使細胞獲得抗性而能在含有Ｇ41 8的選擇性培養基中生長。
Ｇ418的這一選擇特性， 已在基因轉移、基因敲除、抗性篩選以及轉基因動物等方面得以廣泛應用。