Updated Product
Information: Specific Primers for Detection of Tumor Genes
Clinically Confirmed and Highly Specific Primer Sets for Expression
Analysis of Tumor Genes
(Research use only)
> Housekeeping genes: HPRT1, ACTB
> Tumor-associated
genes: CPEB4, DLG2, DUSP6, EIF2S3, ERBB3, EXT2, GRB2, HGF, HMMR, IRF4,
LCK, MCM4, MDM2, MMD, NF1, POLDIP2, RNF4, STAT1, STAT2, WEE1, ZNF264.
- LC Prognosis Primers Kit
(Research use only)
Containing highly
specific primers for CPEB4, DLG2, DUSP6, ERBB3, HGF, HMMR, IRF4, LCK,
MMD, NF1, RNF4, STAT1, STAT2, ZNF264.
- "Advpharma" Analyte Specific Reagents
Containing highly
specific primers for CPEB4, DUSP6, IRF4, MMD, NF1, RNF4, STAT2, ZNF264.
* Reference: A
five-gene signature and clinical outcome in non-small-cell lung cancer.
The NEW ENGLAND JOURNAL of MEDICINE (Chen et al. (2007) 356:11-20)
* Patents: (US)7,955,800, (China) CN102099484A, (Taiwan) I326358
Each vial contains reagent for 48 reactions.
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> Updated Publication List:
Using Prediction Analysis of Microarray, Artificial Neural Network,
Classification and Regression Trees. Disease Markers (Chu et al. (2014))
A Gene Expression Profile of Peripheral Blood in Colorectal Cancer.
Journal of Microbial & Biochemical Technology (Huang et al. (2014) 6:2)
Gene Expression Profiling of Colorectal Tumors and Normal Mucosa by
Microarrays Meta-analysis Gene expression profiling of breast cancer
survivability by pooled cDNA microarray analysis using logistic
regression, artificial neural networks and decision trees. BMC
Bioinformatics (Chou et al. (2013) 14:100)
評估高密度寡核苷酸晶片正規化方法.
Evaluation of Normalization Methods for the High Density Oligonucleotide
Array. Journal of the Chinese Statistical Association (Hwang et al.
(2013) 51: 373-402)G-418.Sulfate
Description:
Aminoglycoside used as a selective agent of transfected mammalian,
yeast, plant and bacteria cells. Resistance is conferred by the
bacterial gene for aminoglycoside-3'-phophotransferase that can be
expressed in eukaryotic cells. Related to Gentamycin.
USAGE:
Geneticin is an aminoglycoside antibiotic similar in structure to
gentamycin. It exhibits toxicity towards both eukaryotic and prokaryotic
cells. The optimal concentration for selection and maintenance must be
determined for each cell line. For bacteria and algae concentrations, 5
ug/ml or less are recommended. Animal cells may require up to 300-500 ug/ml.
Typically, resistance is conferred by one of two dominant genes of
bacterial origin which can be expressed in eukaryotic cells. Cells that
are multiplying will be affected sooner than those that are not. Cells
in log phase may require three to seven days for selection. In general,
concentrations of approximately 400 ug/ml for selection and 200 ug/ml
for maintenance are required for mammalian cells
G418簡介
是一種氨基糖甘類抗生素,在分子遺傳試驗中常被用作抗性篩選。它通過抑制轉做子Tn601,Tn5的基因對原核和真核等細胞產生毒素包括細菌、酵母、植物和哺乳動物細胞,也包括原生動物和蠕蟲。當neo基因被整合進真核細胞DNA後,則能啟動neo基因編碼的序列轉錄為mRNA,從而獲得抗性產物的高效表達,使細胞獲得抗性而能在含有G41
8的選擇性培養基中生長。
G418的這一選擇特性, 已在基因轉移、基因敲除、抗性篩選以及轉基因動物等方面得以廣泛應用。 |