Service
Items
1.
Transient transfection:
Human
lung adenocarcinoma cell A549 or other candidate cell will be transient
transfected with the CRMP-1 promoter/Luc Reporter plasmid using liposome-based
method. The applied DNA construct contains the 1.9 kb promoter region of CRMP-1
gene and the luciferase gene as reporter. The
plasmid base used was the pGL3-basic plasmid (Promega).
2.
Treatment of testing cells with candidate compounds and positive controls.
3.
Measurement of luciferase activities and analysis report.
Applications
Screening
for agents or compounds, which could induce the expression of hCRMP-1.
The increase of luciferase activity measured after the treatment of
CRMP-1 promoter/Luc Reporter Cells indirectly means the up-regulation of hCRMP-1
level.
Background
information
Human
CRMP-1 was reported as “a lung cancer invasion suppressor gene with nerve”
(Ref. 2). Recent discovery showed
that the level of expression of the gene encoding CRMP-1 inversely affects
cancer invasion and metastasis, (i.e., the higher the level of expression, the
lower the incidence of cancer invasion and metastasis) and thus characterized
the CRMP-1 gene as an invasion-suppression gene (Ref. 3-5). Further analysis of
around 50 cases of clinical lung tissue samples using real time-quantitative PCR
techniques found that low-expression patients of CRMP-1 had more advanced
diseases and lymph node metastases, while high-expression patients of CRMP-1 had
a significantly longer disease-free and overall survival period.
“CRMP-1 as an excellent target for lung cancer” was interpreted by
the web “bioportfolio” (Ref. 6).
References
(1)
Steeg (2001) J. Natl. Cancer Inst.
(2001), 93(18): 1364-1365
(2)
Shih et al., J. Natl. Cancer Inst. (2001), 93(18): 1392-1400.
(3)
Chu et al., Am. J. Respir. Cell Mol. Biol. (1997), 17:353-360.
(4)
Shih et al., Clinical & Exper. Metastasis (2003) 20: 69-76).
(5)
http://www.bioportfolio.com/LeadDiscovery/Pubmed-110112.htm
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