●CRMP-1高效能藥理活性篩選
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Cat.
No. SER001
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Service
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Major
service items
1.
Treatment of testing cells with candidate compounds and
positive controls.
2.
Measurement of fluorescent signal and analysis report.
Applications
Screening
for agents or compounds, which could induce the
expression of hCRMP-1.
Human lung adenocarcinoma cell A549 was
transfected stably with the DNA construct, EGFP-hcrmp1,
containing 1.9 kb of promoter region of CRMP-1 gene and
the reporter (GFP) gene.
The promoter fragment was inserted into the
multiple cloning site of pEGFP-1 plasmid (BD
Biosciences; Ref. 1).
Background
information
Human
CRMP-1 was reported as “a lung cancer invasion
suppressor gene with nerve” (Ref. 2).
Recent discovery showed that the level of
expression of the gene encoding CRMP-1 inversely affects
cancer invasion and metastasis, (i.e., the higher the
level of expression, the lower the incidence of cancer
invasion and metastasis) and thus characterized the
CRMP-1 gene as an invasion-suppression gene (Ref. 3-5).
Further
analysis of around 50 cases of clinical lung tissue
samples using real time-quantitative PCR techniques
found that low-expression patients of CRMP-1 had more
advanced diseases and lymph node metastases, while
high-expression patients of CRMP-1 had a significantly
longer disease-free and overall survival period.
“CRMP-1 as an excellent target for lung
cancer” was interpreted by the web “bioportfolio”
(Ref. 6).
References
(1)
pEGFP-1
(BD Biosciences; http://orders.clontech.com/clontech/techinfo/vectors_dis/pEGFP-1.shtml
(2)
Steeg (2001) J. Natl. Cancer Inst. (2001), 93(18):
1364-1365
(3)
Shih et al., J. Natl. Cancer Inst. (2001), 93(18):
1392-1400.
(4)
Chu et al., Am. J. Respir. Cell Mol. Biol. (1997),
17:353-360.
(5)
Shih et al., Clinical & Exper. Metastasis (2003) 20:
69-76).
(6)
CRMP-1 as an excellent target for lung cancer
http://www.bioportfolio.com/LeadDiscovery/Pubmed-110112.htm
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