Major service items

1. Treatment of testing cells with candidate compounds and positive controls.

2. Measurement of fluorescent signal and analysis report.

Applications

        Screening for agents or compounds, which could induce the expression of hCRMP-1.  Human lung adenocarcinoma cell A549 was transfected stably with the DNA construct, EGFP-hcrmp1, containing 1.9 kb of promoter region of CRMP-1 gene and the reporter (GFP) gene.  The promoter fragment was inserted into the multiple cloning site of pEGFP-1 plasmid (BD Biosciences; Ref. 1). 

Background information

      Human CRMP-1 was reported as “a lung cancer invasion suppressor gene with nerve” (Ref. 2).  Recent discovery showed that the level of expression of the gene encoding CRMP-1 inversely affects cancer invasion and metastasis, (i.e., the higher the level of expression, the lower the incidence of cancer invasion and metastasis) and thus characterized the CRMP-1 gene as an invasion-suppression gene (Ref. 3-5). 

Further analysis of around 50 cases of clinical lung tissue samples using real time-quantitative PCR techniques found that low-expression patients of CRMP-1 had more advanced diseases and lymph node metastases, while high-expression patients of CRMP-1 had a significantly longer disease-free and overall survival period.  “CRMP-1 as an excellent target for lung cancer” was interpreted by the web “bioportfolio” (Ref. 6).

References

 (1) pEGFP-1 (BD Biosciences; http://orders.clontech.com/clontech/techinfo/vectors_dis/pEGFP-1.shtml

(2) Steeg (2001) J. Natl. Cancer Inst. (2001), 93(18): 1364-1365

(3) Shih et al., J. Natl. Cancer Inst. (2001), 93(18): 1392-1400.

(4) Chu et al., Am. J. Respir. Cell Mol. Biol. (1997), 17:353-360.

(5) Shih et al., Clinical & Exper. Metastasis (2003) 20: 69-76).

(6) CRMP-1 as an excellent target for lung cancer

http://www.bioportfolio.com/LeadDiscovery/Pubmed-110112.htm