Content

        Phosphate buffer-based system containing additional protein-stabilizing component. 

Applications

        Suitable for manufacturing membrane-based protein array, e.g. antibody array.

Advantages:One solution for two functions - Spotting and Conservation

•   Manufacture process of protein arrays can be reduced.  Once protein spotted or printed using the SPOT buffer, all these proteins could be preserved in dry form.  

• Stabilizing effect: Proteins spotted/immobilized on membrane-based substrate are full active after at least 1.5 years storage at room temperature.  

*** Stability Tests of SPOT Buffer ***

1. Storage environments:  (1) 25℃, 60% humidity; (2) 4℃

2. Sandwich-based Assay:

• Capture antibody: monoclonal anti-mouse IL2 antibody

• Antigen: mouse IL2 recombinant protein

• Detection antibody: Biotin-labeled monoclonal anti-mouse IL2 antibody

3. Normalization of Assay Data: (I: Intensity)

       I spot (stored at 25°C)/ I spot (stored at 4°C)