Protein ID

        Amino acid sequence of purified protein was confirmed by LC/MS/MS analysis and following mapping using search type of MS/MS Ion Search (http://www.matrixscience.com/search_form_select.html).

Applications

          SDS-PAGE, Western Blot, ELISA

Background information

    Human collapsin response mediator protein-1 (hCRMP-1), also named as dihydropyrimidinase related protein-1 (DRP-1), is a 62 kDa phosphoprotein.  hCRMP-1 was originally discovered in the brain tissue and thought to be a brain specific protein involved in the collapsin-induced growth cone collapse during neural development (Ref. 1).

Human CRMP-1 was reported as “a lung cancer invasion suppressor gene with nerve” (Ref. 2).  Recent discovery showed that the level of expression of the gene encoding hCRMP-1 inversely affects cancer invasion and metastasis, (i.e., the higher the level of expression, the lower the incidence of cancer invasion and metastasis) and thus characterized the CRMP-1 gene as an invasion-suppression gene (Ref. 3-5).  Further analysis of around 50 cases of clinical lung tissue samples using real time-quantitative PCR techniques found that low-expression patients of CRMP-1 had more advanced diseases and lymph node metastases, while high-expression patients of CRMP-1 had a significantly longer disease-free and overall survival period.  “CRMP-1 as an excellent target for lung cancer” was interpreted by the web “bioportfolio” (Ref. 6).

References

(1) Torres et al., DNA Res. (1998), 5(6): 393-395.

(2) Steeg (2001) J. Natl. Cancer Inst. (2001), 93(18): 1364-1365

(3) Shih et al., J. Natl. Cancer Inst. (2001), 93(18): 1392-1400.

(4) Chu et al., Am. J. Respir. Cell Mol. Biol. (1997), 17:353-360.

(5) Shih et al., Clinical & Exper. Metastasis (2003) 20: 69-76).

(6) http://www.bioportfolio.com/LeadDiscovery/Pubmed-110112.htm